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five-fold bigger activity than did the WT (Figure 3a). Immunoblotting confirmed which the expression amounts of mutant Aph1 and PS1 have been comparable to stages inside the WT. These effects suggest that γ-secretase Using these two Aph1 mutations show Improved protease activity for application.
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Immunoprecipitants as well as input fraction had been analyzed by immunoblotting working with particular antibodies. The input signifies 25% of your microsomal extract.
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